Genome-wide analysis of a G-quadruplex-specific single-chain antibody that regulates gene expression

نویسندگان

  • Himesh Fernando
  • Sven Sewitz
  • Jeremy Darot
  • Simon Tavaré
  • Julian Leon Huppert
  • Shankar Balasubramanian
چکیده

G-quadruplex nucleic acids have been proposed to play a role in a number of fundamental biological processes that include transcription and translation. We have developed a single-chain antibody that is selective for G-quadruplex DNA over double-stranded DNA, and here show that when it is expressed in human cells, it significantly affects the expression of a wide variety of genes, in a manner that correlates with the presence of predicted G-quadruplexes. We observe cases where gene expression is increased or decreased, and that there are apparent interactions with G-quadruplex motifs at the beginning and end of the genes, and on either strand. The outcomes of this genome-wide study demonstrate that G-quadruplex recognition by the antibody has physiological consequences, and provides insights into some of the complexity associated with G-quadruplex-based regulation.

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عنوان ژورنال:

دوره 37  شماره 

صفحات  -

تاریخ انتشار 2009